Open Science Research Excellence

Open Science Index

Commenced in January 2007 Frequency: Monthly Edition: International Publications Count: 30677


Select areas to restrict search in scientific publication database:
10001853
Assessment of Diagnostic Enzymes as Indices of Heavy Metal Pollution in Tilapia Fish
Abstract:
Diagnostic enzymes like aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were determined as indices of heavy metal pollution in Tilapia guinensis. Three different sets of fishes treated with lead (Pb), iron (Fe) and copper (Cu) were used for the study while a fourth group with no heavy metal served as a control. Fishes in each of the groups were exposed to 2.65mg/l of Pb, 0.85mg/l of Fe and 0.35 mg/l of Cu in aerated aquaria for 96 hours. Tissue fractionation of the liver tissues was carried out and the three diagnostic enzymes (AST, ALT, and ALP) were estimated. Serum levels of the same diagnostic enzymes were also measured. The mean values of the serum enzyme activity for ALP in each experimental group were 19.5±1.62, 29.67±2.17 and 1.15±0.27 IU/L for Pb, Fe and Cu groups compared with 9.99±1.34 IU/L enzyme activity in the control. This result showed that Pb and Fe caused increased release of the enzyme into the blood circulation indicating increased tissue damage while Cu caused a reduction in the serum level as compared with the level in the control group. The mean values of enzyme activity obtained in the liver were 102.14±6.12, 140.17±2.06 and 168.23±3.52 IU/L for Pb, Fe and Cu groups, respectively compared to 91.20±9.42 IU/L enzyme activity for the control group. The serum and liver AST and ALT activities obtained in Pb, Fe, Cu and control groups are reported. It was generally noted that the presence of the heavy metal caused liver tissues damage and consequent increased level of the diagnostic enzymes in the serum.
Digital Object Identifier (DOI):

References:

[1] J. C. Reis. Coping with the waste stream from Drilling for oil. Proceedings, Ecoworld’92 Conference, June, 14-17 in Washington D. C., 1992.
[2] J. I. R. Udotong and O. U. M. John. Spatio- temporal variations in heavy metal concentrations in sediment of QIRE, Nigeria, unpublished, 2015.
[3] J. Tariq, M. Ashraf, M. Jaffar, and M. Af Zal. Pollution status of the Indus. Rivers, Pakistan, through heavy metal and macronutrient contents of fish, sediment and water. Wat. Res., 30: 1337 – 1344, 1996.
[4] I. R. Udotong. Environmental monitoring and effect of petroleum production effluent on some biota of the lower Qua Iboe river estuary. Ph.D thesis, University of Science and Technology, Nkpolu- Oroworukwo, Port-harcourt, Nigeria, 295, 2000.
[5] J. I. R. Udotong. Bioaccumulation and biotoxicity of heavy metals, polychlorinated biphenyls andhydrocarbons in tilapia, periwinkle and oysters from Qua Iboe River estuary. Ph. D thesis, University of calabar, Calabar, Nigeria, P. 95, 2004.
[6] United Nations Conference in Environment and Development (UNCED) Earth Summit. Brazil: UNCED Rio De Janeiro, 1992.
[7] R. Wagemann and D. C. G. Muir. Concentrations of heavy metals and Organochlorines in marine mammals of Northern waters: an overview and evaluation. Can. Tech. Rep. Fish. Aquat. Sci., 1279, 1–97, 1984.
[8] A. C. de Kock, P. B. Best, V. Cockroft and C. Bosama. Persistent organochlorine residues in small cetaceans from the east and west coast of Southern Africa. The Science of the Total Environment, 154, 153 – 162, 1994.
[9] P. D. Abel (Ed.). Water Pollution Biology. Chichester: Elis Horwood Limited, 101- 142pp. 1989.
[10] Metcalf and Eddy. Wastewater Characteristics, Inc: Wastewater Engineering: Treatment Disposal, Reuse. New York: McGraw- Hill, 1995.
[11] E. R. Mancini and C. T. Stilwell. Biotoxicity characterization of a produced water discharge in Wyoming. Journal of Protection Technol., 44(6), 744 – 748, 1992.
[12] J. I. R. Udotong, and O. U. M. John. Histopathological changes in tilapia fish exposed to heavy metals toxicants, unpublished, 2015.
[13] M. Ahsannulah. Acute toxicity of Zinc and Cadmium to seven invertebrate species from Western Port, Victoria. Aust. J. Mar. Fresh wat. Res., 27, 187 –196, 1976.
[14] J. B. Spraque. The ABC’s of pollutant bioassay using fish. In: Biological methods for the assessment of water quality. ASTM Special Technical Publication 528, 6–30, 1973.
[15] IFCC. Expert on enzymes, provisional recommendations on IFCC methods for the measurement of catalytic concentrations of enzymes, part 2. Clin. Chem. Acta, 70, F 19-42, 1986.
[16] M. N. MacSween and K. Whaley. Nutritional disorders: Minerals and trace element (338-354). London: In: Muir’s textbook of pathology. 13th Edition ELBS. Edward Arnold, 1992.
Vol:14 No:09 2020Vol:14 No:08 2020Vol:14 No:07 2020Vol:14 No:06 2020Vol:14 No:05 2020Vol:14 No:04 2020Vol:14 No:03 2020Vol:14 No:02 2020Vol:14 No:01 2020
Vol:13 No:12 2019Vol:13 No:11 2019Vol:13 No:10 2019Vol:13 No:09 2019Vol:13 No:08 2019Vol:13 No:07 2019Vol:13 No:06 2019Vol:13 No:05 2019Vol:13 No:04 2019Vol:13 No:03 2019Vol:13 No:02 2019Vol:13 No:01 2019
Vol:12 No:12 2018Vol:12 No:11 2018Vol:12 No:10 2018Vol:12 No:09 2018Vol:12 No:08 2018Vol:12 No:07 2018Vol:12 No:06 2018Vol:12 No:05 2018Vol:12 No:04 2018Vol:12 No:03 2018Vol:12 No:02 2018Vol:12 No:01 2018
Vol:11 No:12 2017Vol:11 No:11 2017Vol:11 No:10 2017Vol:11 No:09 2017Vol:11 No:08 2017Vol:11 No:07 2017Vol:11 No:06 2017Vol:11 No:05 2017Vol:11 No:04 2017Vol:11 No:03 2017Vol:11 No:02 2017Vol:11 No:01 2017
Vol:10 No:12 2016Vol:10 No:11 2016Vol:10 No:10 2016Vol:10 No:09 2016Vol:10 No:08 2016Vol:10 No:07 2016Vol:10 No:06 2016Vol:10 No:05 2016Vol:10 No:04 2016Vol:10 No:03 2016Vol:10 No:02 2016Vol:10 No:01 2016
Vol:9 No:12 2015Vol:9 No:11 2015Vol:9 No:10 2015Vol:9 No:09 2015Vol:9 No:08 2015Vol:9 No:07 2015Vol:9 No:06 2015Vol:9 No:05 2015Vol:9 No:04 2015Vol:9 No:03 2015Vol:9 No:02 2015Vol:9 No:01 2015
Vol:8 No:12 2014Vol:8 No:11 2014Vol:8 No:10 2014Vol:8 No:09 2014Vol:8 No:08 2014Vol:8 No:07 2014Vol:8 No:06 2014Vol:8 No:05 2014Vol:8 No:04 2014Vol:8 No:03 2014Vol:8 No:02 2014Vol:8 No:01 2014
Vol:7 No:12 2013Vol:7 No:11 2013Vol:7 No:10 2013Vol:7 No:09 2013Vol:7 No:08 2013Vol:7 No:07 2013Vol:7 No:06 2013Vol:7 No:05 2013Vol:7 No:04 2013Vol:7 No:03 2013Vol:7 No:02 2013Vol:7 No:01 2013
Vol:6 No:12 2012Vol:6 No:11 2012Vol:6 No:10 2012Vol:6 No:09 2012Vol:6 No:08 2012Vol:6 No:07 2012Vol:6 No:06 2012Vol:6 No:05 2012Vol:6 No:04 2012Vol:6 No:03 2012Vol:6 No:02 2012Vol:6 No:01 2012
Vol:5 No:12 2011Vol:5 No:11 2011Vol:5 No:10 2011Vol:5 No:09 2011Vol:5 No:08 2011Vol:5 No:07 2011Vol:5 No:06 2011Vol:5 No:05 2011Vol:5 No:04 2011Vol:5 No:03 2011Vol:5 No:02 2011Vol:5 No:01 2011
Vol:4 No:12 2010Vol:4 No:11 2010Vol:4 No:10 2010Vol:4 No:09 2010Vol:4 No:08 2010Vol:4 No:07 2010Vol:4 No:06 2010Vol:4 No:05 2010Vol:4 No:04 2010Vol:4 No:03 2010Vol:4 No:02 2010Vol:4 No:01 2010
Vol:3 No:12 2009Vol:3 No:11 2009Vol:3 No:10 2009Vol:3 No:09 2009Vol:3 No:08 2009Vol:3 No:07 2009Vol:3 No:06 2009Vol:3 No:05 2009Vol:3 No:04 2009Vol:3 No:03 2009Vol:3 No:02 2009Vol:3 No:01 2009
Vol:2 No:12 2008Vol:2 No:11 2008Vol:2 No:10 2008Vol:2 No:09 2008Vol:2 No:08 2008Vol:2 No:07 2008Vol:2 No:06 2008Vol:2 No:05 2008Vol:2 No:04 2008Vol:2 No:03 2008Vol:2 No:02 2008Vol:2 No:01 2008
Vol:1 No:12 2007Vol:1 No:11 2007Vol:1 No:10 2007Vol:1 No:09 2007Vol:1 No:08 2007Vol:1 No:07 2007Vol:1 No:06 2007Vol:1 No:05 2007Vol:1 No:04 2007Vol:1 No:03 2007Vol:1 No:02 2007Vol:1 No:01 2007