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Commenced in January 2007 Frequency: Monthly Edition: International Publications Count: 31311

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Comparative Study on the Effect of Substitution of Li and Mg Instead of Ca on Structural and Biological Behaviors of Silicate Bioactive Glass
In this study, experiments were carried out to achieve a promising multifunctional and modified silicate based bioactive glass (BG). The main aim of the study was investigating the effect of lithium (Li) and magnesium (Mg) substitution, on in vitro bioactivity of substituted-58S BG. Moreover, it is noteworthy to state that modified BGs were synthesized in 60SiO2–(36-x)CaO–4P2O5–(x)Li2O and 60SiO2–(36-x)CaO–4P2O5–(x)MgO (where x = 0, 5, 10 mol.%) quaternary systems, by sol-gel method. Their performance was investigated through different aspects such as biocompatibility, antibacterial activity as well as their effect on alkaline phosphatase (ALP) activity, and proliferation of MC3T3 cells. The antibacterial efficiency was evaluated against methicillin-resistant Staphylococcus aureus bacteria. To do so, CaO was substituted with Li2O and MgO up to 10 mol % in 58S-BGs and then samples were immersed in simulated body fluid up to 14 days and then, characterized by X-ray diffraction, Fourier transform infrared spectroscopy, inductively coupled plasma atomic emission spectrometry, and scanning electron microscopy. Results indicated that this modification led to a retarding effect on in vitro hydroxyapatite (HA) formation due to the lower supersaturation degree for nucleation of HA compared with 58s-BG. Meanwhile, magnesium revealed further pronounced effect. The 3-(4,5 dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) and ALP analysis illustrated that substitutions of both Li2O and MgO, up to 5 mol %, had increasing effect on biocompatibility and stimulating proliferation of the pre-osteoblast MC3T3 cells in comparison to the control specimen. Regarding to bactericidal efficiency, the substitution of either Li or Mg for Ca in the 58s BG composition led to statistically significant difference in antibacterial behaviors of substituted-BGs. Meanwhile, the sample containing 5 mol % CaO/Li2O substitution (BG-5L) was selected as a multifunctional biomaterial in bone repair/regeneration due to the improved biocompatibility, enhanced ALP activity and antibacterial efficiency among all of the synthesized L-BGs and M-BGs.
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[1] F. Gervaso, A. Sannino, G.M. Peretti, The biomaterialist’s task: scaffold biomaterials and fabrication technologies, Joints, 1 (2013) 130.
[2] M. Tahriri, M. Del Monico, A. Moghanian, M.T. Yaraki, R. Torres, A. Yadegari, L. Tayebi, Graphene and its derivatives: Opportunities and challenges in dentistry, Materials Science and Engineering: C, 102 (2019) 171-185.
[3] M.P. Nikolova, M.S. Chavali, Recent advances in biomaterials for 3D scaffolds: A review, Bioactive materials, 4 (2019) 271-292.
[4] M. Kazem-Rostami, A. Moghanian, Hünlich base derivatives as photo-responsive Λ-shaped hinges, Organic Chemistry Frontiers, 4 (2017) 224-228.
[5] M. Mozafari, F. Moztarzadeh, M. Rabiee, M. Azami, N. Nezafati, Z. Moztarzadeh, M. Tahriri, Development of 3D bioactive nanocomposite scaffolds made from gelatin and nano bioactive glass for biomedical applications, Advanced Composites Letters, 19 (2010).
[6] X. Zhang, Y. Yu, D. Jiang, Y. Jiao, Y. Wu, Z. Peng, J. Zhou, J. Wu, Z. Dong, Synthesis and characterization of a bi-functional hydroxyapatite/Cu-doped TiO2 composite coating, Ceramics International, 45 (2019) 6693-6701.
[7] A. Moghanian, F. Sharifianjazi, P. Abachi, E. Sadeghi, H. Jafarikhorami, A. Sedghi, Production and properties of Cu/TiO2 nano-composites, Journal of Alloys and Compounds, 698 (2017) 518-524.
[8] F. Shojaeepour, P. Abachi, K. Purazrang, A.H. Moghanian, Production and properties of Cu/Cr2O3 nano-composites, Powder technology, 222 (2012) 80-84.
[9] E. Kalantari, S.M. Naghib, N.J. Iravani, R. Esmaeili, M.R. Naimi-Jamal, M. Mozafari, Biocomposites based on hydroxyapatite matrix reinforced with nanostructured monticellite (CaMgSiO4) for biomedical application: synthesis, characterization, and biological studies, Materials Science and Engineering: C, 105 (2019) 109912.
[10] H. Shin, S. Jo, A.G. Mikos, Biomimetic materials for tissue engineering, Biomaterials, 24 (2003) 4353-4364.
[11] P.X. Ma, Biomimetic materials for tissue engineering, Advanced drug delivery reviews, 60 (2008) 184-198.
[12] M. Touri, F. Kabirian, M. Saadati, S. Ramakrishna, M. Mozafari, Additive manufacturing of biomaterials− the evolution of rapid prototyping, Advanced Engineering Materials, 21 (2019) 1800511.
[13] S. Kargozar, F. Baino, S. Hamzehlou, M.R. Hamblin, M. Mozafari, Nanotechnology for angiogenesis: opportunities and challenges, Chemical Society Reviews, 49 (2020) 5008-5057.
[14] A.J. Hassiba, M.E. El Zowalaty, G.K. Nasrallah, T.J. Webster, A.S. Luyt, A.M. Abdullah, A.A. Elzatahry, Review of recent research on biomedical applications of electrospun polymer nanofibers for improved wound healing, Nanomedicine, 11 (2016) 715-737.
[15] S. Chen, B. Liu, M.A. Carlson, A.F. Gombart, D.A. Reilly, J. Xie, Recent advances in electrospun nanofibers for wound healing, Nanomedicine, 12 (2017) 1335-1352.
[16] D. Khorsandi, A. Moghanian, R. Nazari, G. Arabzadeh, S. Borhani, Personalized Medicine: Regulation of Genes in Human Skin Ageing, J Allergy Ther, 7 (2016) 2.
[17] M. Norouzi, S.M. Boroujeni, N. Omidvarkordshouli, M. Soleimani, Advances in skin regeneration: application of electrospun scaffolds, Advanced healthcare materials, 4 (2015) 1114-1133.
[18] G. Suarato, R. Bertorelli, A. Athanassiou, Borrowing from Nature: biopolymers and biocomposites as smart wound care materials, Frontiers in bioengineering and biotechnology, 6 (2018) 137.
[19] S. Kargozar, F. Baino, S. Hamzehlou, R.G. Hill, M. Mozafari, Bioactive glasses entering the mainstream, Drug discovery today, 23 (2018) 1700-1704.
[20] J. Yang, T. Long, N.-F. He, Y.-P. Guo, Z.-A. Zhu, Q.-F. Ke, Fabrication of a chitosan/bioglass three-dimensional porous scaffold for bone tissue engineering applications, Journal of Materials Chemistry B, 2 (2014) 6611-6618.
[21] E. Pazos-Ortiz, J.H. Roque-Ruiz, E.A. Hinojos-Márquez, J. López-Esparza, A. Donohué-Cornejo, J.C. Cuevas-González, L.F. Espinosa-Cristóbal, S.Y. Reyes-López, Dose-dependent antimicrobial activity of silver nanoparticles on polycaprolactone fibers against gram-positive and gram-negative bacteria, Journal of Nanomaterials, 2017 (2017).
[22] M. Hosseinnejad, S.M. Jafari, Evaluation of different factors affecting antimicrobial properties of chitosan, International journal of biological macromolecules, 85 (2016) 467-475.
[23] K. Kalantari, A.M. Afifi, H. Jahangirian, T.J. Webster, Biomedical applications of chitosan electrospun nanofibers as a green polymer–Review, Carbohydrate polymers, 207 (2019) 588-600.
[24] R.C. Goy, D.d. Britto, O.B. Assis, A review of the antimicrobial activity of chitosan, Polímeros, 19 (2009) 241-247.
[25] K. Vimala, Y.M. Mohan, K.S. Sivudu, K. Varaprasad, S. Ravindra, N.N. Reddy, Y. Padma, B. Sreedhar, K. MohanaRaju, Fabrication of porous chitosan films impregnated with silver nanoparticles: a facile approach for superior antibacterial application, Colloids and Surfaces B: Biointerfaces, 76 (2010) 248-258.
[26] S. Kumar-Krishnan, E. Prokhorov, M. Hernández-Iturriaga, J.D. Mota-Morales, M. Vázquez-Lepe, Y. Kovalenko, I.C. Sanchez, G. Luna-Bárcenas, Chitosan/silver nanocomposites: Synergistic antibacterial action of silver nanoparticles and silver ions, European Polymer Journal, 67 (2015) 242-251.
[27] N. Nezafati, F. Moztarzadeh, S. Hesaraki, Z. Moztarzadeh, M. Mozafari, Biological response of a recently developed nanocomposite based on calcium phosphate cement and sol–gel derived bioactive glass fibers as substitution of bone tissues, Ceramics International, 39 (2013) 289-297.
[28] M. Aminitabar, M. Amirhosseinian, M. Elsa, Synthesis and in vitro Characterization of a Gel-Derived SiO2-CaO-P2O5-SrO-Li2O Bioactive Glass, International Journal of Chemical and Molecular Engineering, 13 (2019) 296-307.
[29] A. Pazhouheshgar, S.A.S. Vanini, A. Moghanian, The experimental and numerical study of fracture behavior of 58s bioactive glass/polysulfone composite using the extended finite elements method, Materials Research Express, 6 (2019) 095208.
[30] M. Elsa, A. Moghanian, Comparative Study of Calcium Content on in vitro Biological and Antibacterial Properties of Silicon-Based Bioglass, International Journal of Chemical and Molecular Engineering, 13 (2019) 288-295.
[31] A. Moghanian, S. Firoozi, M. Tahriri, A. Sedghi, A comparative study on the in vitro formation of hydroxyapatite, cytotoxicity and antibacterial activity of 58S bioactive glass substituted by Li and Sr, Materials Science and Engineering: C, 91 (2018) 349-360.
[32] A. Moghanian, A. Sedghi, A. Ghorbanoghli, E. Salari, The effect of magnesium content on in vitro bioactivity, biological behavior and antibacterial activity of sol–gel derived 58S bioactive glass, Ceramics international, 44 (2018) 9422-9432.
[33] A. Moghanian, S. Firoozi, M. Tahriri, Characterization, in vitro bioactivity and biological studies of sol-gel synthesized SrO substituted 58S bioactive glass, Ceramics International, 43 (2017) 14880-14890.
[34] A. Moghanian, S. Firoozi, M. Tahriri, Synthesis and in vitro studies of sol-gel derived lithium substituted 58S bioactive glass, Ceramics International, 43 (2017) 12835-12843.
[35] A. Moghanian, A. Ghorbanoghli, M. Kazem‐Rostami, A. Pazhouheshgar, E. Salari, M. Saghafi Yazdi, T. Alimardani, H. Jahani, F. Sharifian Jazi, M. Tahriri, Novel antibacterial Cu/Mg‐substituted 58S‐bioglass: Synthesis, characterization and investigation of in vitro bioactivity, International Journal of Applied Glass Science, (2019).
[36] A. Moghanian, M. Zohourfazeli, M.H.M. Tajer, The effect of zirconium content on in vitro bioactivity, biological behavior and antibacterial activity of sol-gel derived 58S bioactive glass, Journal of Non-Crystalline Solids, 546 (2020) 120262.
[37] Z. Hajifathali, M. Amirhosseinian, The Effect of Substitution of CaO/MgO and CaO/SrO on in vitro Bioactivity of Sol-Gel Derived Bioactive Glass, International Journal of Biomedical and Biological Engineering, 13 (2019) 279-287.
[38] A. Moghanian, M. Zohourfazeli, Comparative study on in vitro, physico-chemical and antibacterial properties of 58S and 68S bioactive glasses synthesized by sol-gel method, 13 (2020) 17-30.
[39] S. Kargozar, F. Baino, S. Hamzehlou, R.G. Hill, M. Mozafari, Bioactive glasses: sprouting angiogenesis in tissue engineering, Trends in biotechnology, 36 (2018) 430-444.
[40] N. Nezafati, F. Moztarzadeh, S. Hesaraki, M. Mozafari, Synergistically reinforcement of a self-setting calcium phosphate cement with bioactive glass fibers, Ceramics International, 37 (2011) 927-934.
[41] M. Mozafari, F. Moztarzadeh, Synthesis, characterization and biocompatibility evaluation of sol–gel derived bioactive glass scaffolds prepared by freeze casting method, Ceramics International, 40 (2014) 5349-5355.
[42] M. Zohourfazeli, M.H.M. Tajer, A. Moghanian, Comprehensive investigation on multifunctional properties of zirconium and silver co-substituted 58S bioactive glass, Ceramics International, (2020).
[43] A. Moghanian, M. Zohourfazeli, Investigation the In Vitro and Bactericidal Properties of Magnesium and Copper Containing Bioactive Glasses, Journal of Advanced Materials and Technologies, 9 (2020) 19-33.
[44] A. Pazhouheshgar, A. Moghanian, S.A. Sadough Vanini, The Extended Finite Element Method Numerical and Experimental Analysis of Mechanical Behavior of Polysulfone/58s Bioactive Glass Synthesized through Solvent Casting Method, Modares Mechanical Engineering, 20 (2020) 2061-2073.
[45] S. Kargozar, F. Baino, S. Banijamali, M. Mozafari, Synthesis and physico-chemical characterization of fluoride (F)-and silver (Ag)-substituted sol-gel mesoporous bioactive glasses, Biomedical Glasses, 5 (2019) 185-192.
[46] H. Hu, Y. Tang, L. Pang, C. Lin, W. Huang, D. Wang, W. Jia, Angiogenesis and full-thickness wound healing efficiency of a copper-doped borate bioactive glass/poly (lactic-co-glycolic acid) dressing loaded with vitamin E in vivo and in vitro, ACS applied materials & interfaces, 10 (2018) 22939-22950.
[47] D. Moura, M. Souza, L. Liverani, G. Rella, G. Luz, J. Mano, A. Boccaccini, Development of a bioactive glass-polymer composite for wound healing applications, Materials Science and Engineering: C, 76 (2017) 224-232.
[48] S. Pourshahrestani, E. Zeimaran, N.A. Kadri, N. Gargiulo, H.M. Jindal, S.V. Naveen, S.D. Sekaran, T. Kamarul, M.R. Towler, Potency and cytotoxicity of a novel gallium-containing mesoporous bioactive glass/chitosan composite scaffold as hemostatic agents, ACS applied materials & interfaces, 9 (2017) 31381-31392.
[49] A. Moghanian, R. Portillo‐Lara, E. Shirzaei Sani, H. Konisky, S.H. Bassir, N. Annabi, Synthesis and characterization of osteoinductive visible light‐activated adhesive composites with antimicrobial properties, Journal of tissue engineering and regenerative medicine, 14 (2020) 66-81.
[50] Y.-F. Goh, A.Z. Alshemary, M. Akram, M.R.A. Kadir, R. Hussain, In vitro characterization of antibacterial bioactive glass containing ceria, Ceramics International, 40 (2014) 729-737.
[51] M. Gholipourmalekabadi, M. Sameni, A. Hashemi, F. Zamani, A. Rostami, M. Mozafari, Silver-and fluoride-containing mesoporous bioactive glasses versus commonly used antibiotics: Activity against multidrug-resistant bacterial strains isolated from patients with burns, Burns, 42 (2016) 131-140.
[52] C. Wu, Y. Zhou, M. Xu, P. Han, L. Chen, J. Chang, Y. Xiao, Copper-containing mesoporous bioactive glass scaffolds with multifunctional properties of angiogenesis capacity, osteostimulation and antibacterial activity, Biomaterials, 34 (2013) 422-433.
[53] P. Chavoshnejad, M.J. Razavi, Effect of the Interfiber Bonding on the Mechanical Behavior of Electrospun Fibrous Mats, Scientific Reports, 10 (2020) 1-10.
[54] K.K. Balan, V. Sivanesan, N. Moorthy, D. Budhhan, S. Jeyaseelan, S. Sundaramoorthy, Effect of thickness of mat and testing parameters on tensile strength variability of electrospun nanofibrous mat, Materials Today: Proceedings, 3 (2016) 1320-1329.
[55] J. Jordan, K.I. Jacob, R. Tannenbaum, M.A. Sharaf, I. Jasiuk, Experimental trends in polymer nanocomposites—a review, Materials science and engineering: A, 393 (2005) 1-11.
[56] T. Suchý, M. Šupová, M. Bartoš, R. Sedláček, M. Piola, M. Soncini, G.B. Fiore, P. Sauerová, M.H. Kalbáčová, Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?, Journal of Materials Science: Materials in Medicine, 29 (2018) 20.
[57] A. Saatchi, A.R. Arani, A. Moghanian, M. Mozafari, Synthesis and characterization of electrospun cerium-doped bioactive glass/chitosan/polyethylene oxide composite scaffolds for tissue engineering applications, Ceramics International, (2020).
[58] C.E.G. Garcia, F.A.S. Martínez, F. Bossard, M. Rinaudo, Biomaterials based on electrospun chitosan. Relation between processing conditions and mechanical properties, Polymers, 10 (2018) 257.
[59] P. Sangsanoh, P. Supaphol, Stability improvement of electrospun chitosan nanofibrous membranes in neutral or weak basic aqueous solutions, Biomacromolecules, 7 (2006) 2710-2714.
[60] S. Mengistu Lemma, F. Bossard, M. Rinaudo, Preparation of pure and stable chitosan nanofibers by electrospinning in the presence of poly (ethylene oxide), International journal of molecular sciences, 17 (2016) 1790.
[61] P. Bösiger, I.M. Richard, L. Le Gat, B. Michen, M. Schubert, R.M. Rossi, G. Fortunato, Application of response surface methodology to tailor the surface chemistry of electrospun chitosan-poly (ethylene oxide) fibers, Carbohydrate polymers, 186 (2018) 122-131.
[62] A.N. Sohi, H. Naderi-Manesh, M. Soleimani, S. Mirzaei, M. Delbari, M. Dodel, Influence of chitosan molecular weight and poly (ethylene oxide): Chitosan proportion on fabrication of chitosan based electrospun nanofibers, Polymer Science, Series A, 60 (2018) 471-482.
[63] B.K. Gu, S.J. Park, M.S. Kim, C.M. Kang, J.-I. Kim, C.-H. Kim, Fabrication of sonicated chitosan nanofiber mat with enlarged porosity for use as hemostatic materials, Carbohydrate Polymers, 97 (2013) 65-73.
[64] D. Kozon, K. Zheng, E. Boccardi, Y. Liu, L. Liverani, A.R. Boccaccini, Synthesis of monodispersed Ag-doped bioactive glass nanoparticles via surface modification, Materials, 9 (2016) 225.
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