Effects of Adding Different Levels of Anaerobic Fungi on Cellulase Activity of Ostrich Digestive Tract-s Microorganisms under in Vitro Condition
the objective of this study is to measure the levels of
cellulas activity of ostrich GI microorganisms, and comparing it with
the levels of cellulas activity of rumen-s microorganisms, and also to
estimate the probability of increasing enzyme activity with injecting
different dosages (30%, 50% and 70%) of pure anaerobic goat rumen
fungi. The experiment was conducted in laboratory and under a
complete anaerobic condition (in vitro condition). 40 ml of
“CaldWell" medium and 1.4g wheat straw were placed in incubator
for an hour. The cellulase activity of ostrich microorganisms was
compared with other treatments, and then different dosages (30%,
50% and 70%) of pure anaerobic goat rumen fungi were injected to
ostrich microorganism-s media. Due to the results, cattle and goat
with 2.13 and 2.08 I.U (international units) respectively showed the
highest activity and ostrich with 0.91 (I.U) had the lowest cellulose
activity (p < 0.05). Injecting 30% and 50% of anaerobic fungi had no
significant incensement in enzyme activity, but with injecting 70% of
rumen fungi to ostrich microorganisms culture a significant increase
was observed 1.48 I.U. (p < 0.05).
 M. Chamani, " Separation and identification of anaerobic fungi in the
rumen of sheep and goats from different climates of Iran", PhD thesis
of Animal Science, Science and Research Branch ,Islamic Azad
University, 2002, 146601.
 A. A. Aganga, and U. J. Omphile, "Ostrich feeding and nutrition(
Review Article)," Pakistan Journal of animal science, 2003, 12: 601-
 D. R. Cald well and M.P. Bryant, "Medium without rumen fluid for
nonselective enumeration and isolation of rumen bacteria", 1966.
 G. Cooper and M. Khalid, "Anatomy and Physiology of the
gastrointestinal tract and growth carve of ostrich" Review Article,
Animal Science Journal, 2004, 75: 491-498
 V. Fievez and H.F. Banzam, "Evidence for reductive Acetogenesis and
its nutritional significance in ostrich hind gut as estimated from in vitro
incubation", Applied physiology journal, 2001, 18: 271-280.
 F. W. Huchzermeyer, "Diseases of ostrich and other ratities", Text
book published by MAC IMAGE ISBN , 1984, 1-86849-103.
 D. N Kamra, "Rumen microbial ecosystem", Review Article, Journal
of Indian veterinary Research institute, 2000,13: 199-202
 E. T. Moran , "Anatomy, Microbial and fiber: small versus large
intestine", Poultry science journal, 2006, 15: 156-160.
 N. Ozcan and E. Oskose, "A study on cellulytic and hemicellulolytic
enzyme of anaerobic GI. System microorganisms", Turkish journal of
vet animal science, 2005, 25: 703-709.
 M Rezaeian and G. W. Beakes, "Relative fibrolytic activities of
anaerobic rumen fungi on untreated and sodium hydroxide treated
barley straw in in vitro culture", Journal of Microbiology science, 2005,
 A. A. Safari Sinegani and S. Hajrasuliha and H. Shariatmadarie,
"Biodegradation of some agricultural residue by fungi in agitated
submerged culture", African Journal of biotechnology, 2005, 4: 158-