Open Science Research Excellence

Open Science Index

Commenced in January 2007 Frequency: Monthly Edition: International Publications Count: 30569

Select areas to restrict search in scientific publication database:
Identification and Classification of Gliadin Genes in Iranian Diploid Wheat
Wheat is the first and the most important grain of the world and its bakery property is due to glutenin and gliadin qualities. Wheat seed proteins were divided into four groups according to solubility including albumin, globulin, glutenin and prolamin or gliadin. Gliadins are major components of the storage proteins in wheat endosperm. It seems that little information is available about gliadin genes in Iranian wild relatives of wheat. Thus, the aim of this study was the evaluation of the wheat wild relatives collected from different origins of Zagros Mountains in Iran, in terms of coding gliadin genes using specific primers. For this, forty accessions of Triticum boeoticum and Triticum urartu were selected for this study. For each accession, genomic DNA was extracted and PCRs were performed in total volumes of 15 μl. The amplification products were separated on 1.5% agarose gels. In results, for Gli-2A locus three allelic variants were detected by Gli-2As primer pairs. The sizes of PCR products for these alleles were 210, 490 and 700 bp. Only five (13%) and two accessions (5%) produced 700 and 490 bp fragments when their DNA was amplified with the Gli.As.2 primer pairs. However, 93% of the accessions carried allele 210 bp, and only 8% did not any product for this marker. Therefore, these germplasm could be used as rich gene pool to broaden the genetic base of bread wheat.
Digital Object Identifier (DOI):


[1] B. L. Johnson, H. S. Dhaliwal, “Reproductive isolation of Triticum boeoticum and Triticum urartu and the origin of the tetraploid wheats” American Journal of Botany, 1976, 65: 907-918.
[2] V. F. Dorofeev, A. A. Filatenko, E. F. Migushova, R.A. Udaczin and M. M. Jakubziner, “Wheat” 1979, vol. 1. In: V.F. Dorofeev, O.N. Korovina, (eds.), Flora of Cultivated Plants. Leningrad, Russia.
[3] J. Dvorak, “Transfer of leaf rust resistance from Aegilops speltoides to Triticum aestivum” Canadian Journal of Genetics and Cytology, 1977, 19: 133-141.
[4] B. Kilian, H. Ozkan, O. Deusch, S. Effgen, A. Brandolini, J. Kohl, W. Martin, F. Salamini, “ Independent wheat B and G genome origins in outcrossing Aegilops progenitor haplotypes. Molecular Biology and Evolution, 2007, 24: 217-227.
[5] M. Heun, R. Schafer-Pregl, D. Klawan, R. Castagna, M. Accerbi, B. Borghi, F. Salamini, “Site of Einkorn Wheat Domestication Identified by DNA Fingerprinting” Science, 1997, 278: 1312–1314.
[6] P. L. Weegels, R.J. Hamer, J. D. Schofield “Functional properties of wheat glutenin” Cereal Sci. 1996, 23: 1-17.
[7] P. R. Shewry, N. G. Halford, A. S. Tatham “High molecular weight subunits of wheat glutenin” J. Cereal Sci. 1992, 15: 105-120.
[8] S. Masci, R. Ovidio, D. Lafiandra, D.D. Kasarda “Characterization of a low molecular weight glutenin subunit gene from bread wheat and the corresponding protein that represents a major subunit of the glutenin polymer” Plant Physiology, 1998, 118: 1147-1158.
[9] K. Kawaura, K., Mochida, Y. Ogihara, “ Expression Profile of Two Storage-Protein Gene Families in Hexaploid Wheat Revealed by Large- Scale Analysis of Expressed Sequence Tags” Plant Physiology, 2005, 139:1870-1880.
[10] M.W. Van Slageren, “Wild wheats. A monograph of Aegilops L. and Amblyopyrum (Jaub. & Spach) Eig (Poaceae)” Wageningen Agricultural University, 1994, pp. 512.
[11] S. Piccolo, A. Alfonzo, G. Conigliaro, G. Moschetti, S. Burruano, A. Barone, “A simple and rapid DNA extraction method from leaves of grapevine suitable for polymerase chain reaction analysis” African Journal of Biotechnology, 2012, 11: 10305-10309.
[12] H. Long, Y. Wei, Z.H. Yan, B. Baum, E. Nevo, Y.L. Zheng, “Classification of wheat low-molecular-weight glutenin subunit genes and its chromosome assignment by developing LMW-GS group-specific primers” Theoretical and Applied Genetics, 2005, 111: 1251-1259.
[13] K. Kawaura, K. Mochida, Y. Ogihara, “Expression Profile of Two Storage-Protein Gene Families in Hexaploid Wheat Revealed by Large- Scale Analysis of Expressed Sequence Tags” Plant Physiology, 2005, 139:1870-1880.
[14] M. Zhao-cai, Q. Chen, Y. Zheng, “Allelic identification and genetic diversity at Gli-A1 and Gli-A2 loci in Einkorn wheat” International Journal of Agricultural Research, 2006, 1: 100-107.
[15] L. Wang, G. Li, R.J. Penea, X. Xia and Z. He, “Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.)” Journal of Cereal Science, 2010, 51: 305-312.
[16] S. Sharma, S. Ram, R. Gupta, I. Sharma, “Development of functional marker for distinguishing Glu-B3b allele of LMW-GS found in Indian common wheat cultivars” Journal of Cereal Science, 2013, 57: 245-248.
[17] Y. Q. Gu, C. Crossman, X. Kong, M. Luo, F.M. You, D. Coleman-Derr, J. Dubcovsky, O.D. Anderson, “Genomic organization of the complex alpha-gliadin gene loci in wheat” Theoretical and Applied Genetics, 2004, 109: 648-657.
Vol:14 No:08 2020Vol:14 No:07 2020Vol:14 No:06 2020Vol:14 No:05 2020Vol:14 No:04 2020Vol:14 No:03 2020Vol:14 No:02 2020Vol:14 No:01 2020
Vol:13 No:12 2019Vol:13 No:11 2019Vol:13 No:10 2019Vol:13 No:09 2019Vol:13 No:08 2019Vol:13 No:07 2019Vol:13 No:06 2019Vol:13 No:05 2019Vol:13 No:04 2019Vol:13 No:03 2019Vol:13 No:02 2019Vol:13 No:01 2019
Vol:12 No:12 2018Vol:12 No:11 2018Vol:12 No:10 2018Vol:12 No:09 2018Vol:12 No:08 2018Vol:12 No:07 2018Vol:12 No:06 2018Vol:12 No:05 2018Vol:12 No:04 2018Vol:12 No:03 2018Vol:12 No:02 2018Vol:12 No:01 2018
Vol:11 No:12 2017Vol:11 No:11 2017Vol:11 No:10 2017Vol:11 No:09 2017Vol:11 No:08 2017Vol:11 No:07 2017Vol:11 No:06 2017Vol:11 No:05 2017Vol:11 No:04 2017Vol:11 No:03 2017Vol:11 No:02 2017Vol:11 No:01 2017
Vol:10 No:12 2016Vol:10 No:11 2016Vol:10 No:10 2016Vol:10 No:09 2016Vol:10 No:08 2016Vol:10 No:07 2016Vol:10 No:06 2016Vol:10 No:05 2016Vol:10 No:04 2016Vol:10 No:03 2016Vol:10 No:02 2016Vol:10 No:01 2016
Vol:9 No:12 2015Vol:9 No:11 2015Vol:9 No:10 2015Vol:9 No:09 2015Vol:9 No:08 2015Vol:9 No:07 2015Vol:9 No:06 2015Vol:9 No:05 2015Vol:9 No:04 2015Vol:9 No:03 2015Vol:9 No:02 2015Vol:9 No:01 2015
Vol:8 No:12 2014Vol:8 No:11 2014Vol:8 No:10 2014Vol:8 No:09 2014Vol:8 No:08 2014Vol:8 No:07 2014Vol:8 No:06 2014Vol:8 No:05 2014Vol:8 No:04 2014Vol:8 No:03 2014Vol:8 No:02 2014Vol:8 No:01 2014
Vol:7 No:12 2013Vol:7 No:11 2013Vol:7 No:10 2013Vol:7 No:09 2013Vol:7 No:08 2013Vol:7 No:07 2013Vol:7 No:06 2013Vol:7 No:05 2013Vol:7 No:04 2013Vol:7 No:03 2013Vol:7 No:02 2013Vol:7 No:01 2013
Vol:6 No:12 2012Vol:6 No:11 2012Vol:6 No:10 2012Vol:6 No:09 2012Vol:6 No:08 2012Vol:6 No:07 2012Vol:6 No:06 2012Vol:6 No:05 2012Vol:6 No:04 2012Vol:6 No:03 2012Vol:6 No:02 2012Vol:6 No:01 2012
Vol:5 No:12 2011Vol:5 No:11 2011Vol:5 No:10 2011Vol:5 No:09 2011Vol:5 No:08 2011Vol:5 No:07 2011Vol:5 No:06 2011Vol:5 No:05 2011Vol:5 No:04 2011Vol:5 No:03 2011Vol:5 No:02 2011Vol:5 No:01 2011
Vol:4 No:12 2010Vol:4 No:11 2010Vol:4 No:10 2010Vol:4 No:09 2010Vol:4 No:08 2010Vol:4 No:07 2010Vol:4 No:06 2010Vol:4 No:05 2010Vol:4 No:04 2010Vol:4 No:03 2010Vol:4 No:02 2010Vol:4 No:01 2010
Vol:3 No:12 2009Vol:3 No:11 2009Vol:3 No:10 2009Vol:3 No:09 2009Vol:3 No:08 2009Vol:3 No:07 2009Vol:3 No:06 2009Vol:3 No:05 2009Vol:3 No:04 2009Vol:3 No:03 2009Vol:3 No:02 2009Vol:3 No:01 2009
Vol:2 No:12 2008Vol:2 No:11 2008Vol:2 No:10 2008Vol:2 No:09 2008Vol:2 No:08 2008Vol:2 No:07 2008Vol:2 No:06 2008Vol:2 No:05 2008Vol:2 No:04 2008Vol:2 No:03 2008Vol:2 No:02 2008Vol:2 No:01 2008
Vol:1 No:12 2007Vol:1 No:11 2007Vol:1 No:10 2007Vol:1 No:09 2007Vol:1 No:08 2007Vol:1 No:07 2007Vol:1 No:06 2007Vol:1 No:05 2007Vol:1 No:04 2007Vol:1 No:03 2007Vol:1 No:02 2007Vol:1 No:01 2007