Open Science Research Excellence

Open Science Index

Commenced in January 2007 Frequency: Monthly Edition: International Publications Count: 31106

Select areas to restrict search in scientific publication database:
Influence of Cyperus rotundus Active Principles Inhibit Viral Multiplication and Stimulate Immune System in Indian White Shrimp Fenneropenaeus indicus against White Spot Syndrome Virus Infection
The rhizome of Java grass, Cyperus rotundus was extracted different organic polar and non-polar solvents and performed the in vitro antiviral and immunostimulant activities against White Spot Syndrome Virus (WSSV) and Vibrio harveyi respectively. Based on the initial screening the ethyl acetate extract of C. rotundus was strong activities and further it was purified through silica column chromatography and the fractions were screened again for antiviral and immunostimulant activity. Among the different fractions screened against the WSSV and V. harveyi, the fractions, FIII to FV had strong activities. In order to study the in vivo influence of C. rotundus, the fractions (F-III to FV) were pooled and delivered to the F. indicus through artificial feed for 30 days. After the feeding trail the experimental and control diet fed F. indicus were challenged with virulent WSSV and studied the survival, molecular diagnosis, biochemical, haematological, and immunological parameters. Surprisingly, the pooled fractions (F-IV to FVI) incorporated diets helped to significantly (P<0.01) suppressed viral multiplication, showed significant (P<0.01) differences in protein and glucose levels, improved total haemocyte count (THC), coagulase activity, significantly increased (P <= 0.001) prophenol oxidase and intracellular superoxide anion production compared to the control shrimps. Based on the results, C. rotundus extracts effectively suppressed WSSV multiplication and improve the immune system in F. indicus against WSSV infection and this knowledge will helps to develop novel drugs from C. rotundus against WSSV.
Digital Object Identifier (DOI):


[1] T. Citarasu, R. R. J. Sekar, M. M. Babu and M.P. Marian, “Developing Artemia enriched herbal diet for producing quality larvae in Penaeus monodon.” Asian Fisheries Science, vol 15, 2002, pp. 21-32.
[2] FAO, “Report of the expert consultation on the proposed subcommittee on aquaculture of the committee on fisheries” Rome, FAO, 2000, p. 36.
[3] T. Flegel, W. S. Boonyaratpalin and B. Withyachumnarnkul, “Progress in research on yellow-head virus and white spot virus in Thailand” In: Asian Fisheries Society (Eds. T.W Flegel, I.H. MacRae), Manila, 1997. pp. 285-96.
[4] T. Citarasu, K.V. Ramalingam, R.R.J. Sekar, M. M. Babu and M.P. Marian, “Influence of the antibacterial herbs, Solanum trilobatum, Andrographis paniculata and Psoralea corylifolia on the survival, growth and bacterial load of Penaeus monodon post larvae.” Aquaculture International, vol 11, 2003, pp.583-95.
[5] T. Citarasu, V. Sivaram, G. Immanuel, N. Rout and V. Murugan, “Influence of selected Indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp, Penaeus monodon with reference to haematological, biochemical and immunological changes.” Fish and Shellfish Immunology, vol. 21, 2006, pp.372-84.
[6] T. Citarasu, “Natural antimicrobials.” In: Aquaculture infective disease in aquaculture: prevention and control.(Eds. B. Austin) Woodhead Publishing Ltd, United Kingdom, 2012. p. 419.
[7] P. Smith, M. P. Hiney and O. B. Samuelsen, “Bacterial resistance to antimicrobial agent used in fish farming: a critical evaluation of method and meaning.” Annual Review of Fish Diseases, vol 4, 1994, pp. 273- 313.
[8] T. Citarasu, “Herbal biomedicines - a new opportunity to aquaculture industry.” Aquaculture International, vol 18, 2010, pp. 403-14.
[9] G. Balasubramanian, M. Sarathi, S. Rajesh Kumar and A.S. Sahul Hameed. “Screening the antiviral activity of Indian medicinal plants against white spot syndrome virus in shrimp.” Aquaculture, vol 263,2007, pp. 15-9.
[10] P. Raja Rajeswari, S. Velmurugan, M. M. Babu, K. Kesavan and T. Citarasu, “A study on the influence of selected Indian herbal active principles on enhancing the immune system in Fenneropenaeus indicus against Vibrio harveyi infection.” Aquaculture International, vol 20 2012, pp. 1009–1020.
[11] M. Boonyaratpalin, “Nutritional requirements of grouper Epinephelus.” In: The proceedings of grouper culture. Thailand: National Institute of Coastal Aquaculture. Department of Fisheries, 1993. p. 50-55.
[12] R. Namita, S. Kumar, S. Jaganmohan and V. Murugan, “DNA vaccines encoding viral envelope proteins confer protective immunity against WSSV in black tiger shrimp.” Vaccine, vol 25, 2007, pp. 2778-86.
[13] C.P. Malik, M.B.Singh, “Plant enzymology and histoenzymology.” Kalyani Publishers, New Delhi, 1980, p. 278.
[14] K. N. Sachdev, “Clinical pathology and bacteriology.” Jaypee Brother, New Delhi, 1983, p. 133.
[15] G. Le Moullac, M. Le Groumellec, D. Ansquer, S. Froissard, P. Aquacop Levy, “Haematological and phenoloxidase activity changes in the shrimp Penaeus stylirostris in relation with the moult cycle: protection against vibriosis.” Fish and Shellfish Immunology, vol 7, 1997, pp.227-34.
[16] L. Hagerman, “Haemocyanin concentration of juvenile lobster (Homarus gammarus) in relation to molting cycle and feeding conditions.” Marine Biology, vol 77, 1983, pp. 11-17.
[17] K. Söderhäll and V.J. Smith, “Separation of haemocyte populations of Carcinus maenus and other marine decapods and phenoloxidase distribution.” Developmental and Comparative Immunology, vol 7, 1983, PP. 229–239
[18] Y.L. Song and Y.T. Hsieh, “Immunostimulation of tiger shrimp (Penaeus monodon) haemocytes for generation of microbiocidal substances: analysis of reactive oxygen species.” Developmental and Comparative Immunology, vol 18, 1994, pp. 201-209.
[19] S. Direkbusarakom, A. Herunsalee, S. Boonyaratpalin, Y. Danayadol and U. Aekpanithanpong, “Effect of Phyllanthus spp against yellow head baculovirus infection in black tiger shrimp, Penaeus monodon.” In: Disease in Asian aquaculture II. (Eds. M. Shariff, J.R. Arthur, R.P. Subasinghe), Fish Health Section, Asian Fisheries Society, Manila, 1993. p. 81-88.
[20] M. Minomol, “Culture of Gold fish Carassius auratus using medicinal plants having immunostimulant characteristics.” M. Phil Dissertation, M. S. University, Tirunelveli, India, 2005
[21] A. Yogeeswaran, S. Velmurugan, S.M.J. Punitha, M.M. Babu, T. Selvaraj, T. Kumaran and T. Citarasu, “Protection of Penaeus monodon against white spot syndrome virus by inactivated vaccine with herbal immunostimulants.” Fish and Shellfish Immunology, vol 32(6), 2012, pp. 1058-67.
[22] C.F. Lo, C.H. Ho, C.H.Chen, K.F. Liu, Y.L. Chiu, P.Y. Yeh, “Detection and tissue tropism of white spot syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organ.” Disease in Aquatic Organism, vol 30, 1997, pp. 53- 72.
[23] M.R. Hall, E.H and Van Ham, “The effect of different types of stress on blood glucose in the giant tiger prawn Penaeus monodon.” Journal of World Aquaculture Society, 29, 1998, 290- 9.
[24] M. Maeda, T. Itami, M. Kondo, O. Henning, Y. Takahashi and I. Hirono, “Characteristics of penaeid rod shaped DNA virus of kuruma shrimp.” In: New approaches to viral disease of aquatic animals, NRIA International Workshop. Nansei, Mie, Japan: National Research Institute of Aquaculture, 1997, p. 218-28.
[25] Yoganandhan K, Thirupathi S and Sahul Hameed AS. Biochemical, physiological and hematological changes in white spot syndrome virus infected shrimp, Penaeus indicus. Aquaculture 2002;62:1e11.
[26] F. Bindhu, S. Velmurugan, M. B. S. Donio, M. Michaelbabu and T. Citarasu, “Influence of Agathi grandiflora active principles inhibit viral multiplication and stimulate immune system in Indian white shrimp Fenneropenaeus indicus against white spot syndrome virus infection.” Fish and Shellfish Immunology, vol 41, 2014, pp. 482-492
[27] M.W. Johansson, P. Keyser, K. Sritunyalucksana and K. Soderhall, “Crustacean haemocytes and haematopoiesis.” Aquaculture, VOL 191, 2000, pp. 45-52.
Vol:15 No:03 2021Vol:15 No:02 2021Vol:15 No:01 2021
Vol:14 No:12 2020Vol:14 No:11 2020Vol:14 No:10 2020Vol:14 No:09 2020Vol:14 No:08 2020Vol:14 No:07 2020Vol:14 No:06 2020Vol:14 No:05 2020Vol:14 No:04 2020Vol:14 No:03 2020Vol:14 No:02 2020Vol:14 No:01 2020
Vol:13 No:12 2019Vol:13 No:11 2019Vol:13 No:10 2019Vol:13 No:09 2019Vol:13 No:08 2019Vol:13 No:07 2019Vol:13 No:06 2019Vol:13 No:05 2019Vol:13 No:04 2019Vol:13 No:03 2019Vol:13 No:02 2019Vol:13 No:01 2019
Vol:12 No:12 2018Vol:12 No:11 2018Vol:12 No:10 2018Vol:12 No:09 2018Vol:12 No:08 2018Vol:12 No:07 2018Vol:12 No:06 2018Vol:12 No:05 2018Vol:12 No:04 2018Vol:12 No:03 2018Vol:12 No:02 2018Vol:12 No:01 2018
Vol:11 No:12 2017Vol:11 No:11 2017Vol:11 No:10 2017Vol:11 No:09 2017Vol:11 No:08 2017Vol:11 No:07 2017Vol:11 No:06 2017Vol:11 No:05 2017Vol:11 No:04 2017Vol:11 No:03 2017Vol:11 No:02 2017Vol:11 No:01 2017
Vol:10 No:12 2016Vol:10 No:11 2016Vol:10 No:10 2016Vol:10 No:09 2016Vol:10 No:08 2016Vol:10 No:07 2016Vol:10 No:06 2016Vol:10 No:05 2016Vol:10 No:04 2016Vol:10 No:03 2016Vol:10 No:02 2016Vol:10 No:01 2016
Vol:9 No:12 2015Vol:9 No:11 2015Vol:9 No:10 2015Vol:9 No:09 2015Vol:9 No:08 2015Vol:9 No:07 2015Vol:9 No:06 2015Vol:9 No:05 2015Vol:9 No:04 2015Vol:9 No:03 2015Vol:9 No:02 2015Vol:9 No:01 2015
Vol:8 No:12 2014Vol:8 No:11 2014Vol:8 No:10 2014Vol:8 No:09 2014Vol:8 No:08 2014Vol:8 No:07 2014Vol:8 No:06 2014Vol:8 No:05 2014Vol:8 No:04 2014Vol:8 No:03 2014Vol:8 No:02 2014Vol:8 No:01 2014
Vol:7 No:12 2013Vol:7 No:11 2013Vol:7 No:10 2013Vol:7 No:09 2013Vol:7 No:08 2013Vol:7 No:07 2013Vol:7 No:06 2013Vol:7 No:05 2013Vol:7 No:04 2013Vol:7 No:03 2013Vol:7 No:02 2013Vol:7 No:01 2013
Vol:6 No:12 2012Vol:6 No:11 2012Vol:6 No:10 2012Vol:6 No:09 2012Vol:6 No:08 2012Vol:6 No:07 2012Vol:6 No:06 2012Vol:6 No:05 2012Vol:6 No:04 2012Vol:6 No:03 2012Vol:6 No:02 2012Vol:6 No:01 2012
Vol:5 No:12 2011Vol:5 No:11 2011Vol:5 No:10 2011Vol:5 No:09 2011Vol:5 No:08 2011Vol:5 No:07 2011Vol:5 No:06 2011Vol:5 No:05 2011Vol:5 No:04 2011Vol:5 No:03 2011Vol:5 No:02 2011Vol:5 No:01 2011
Vol:4 No:12 2010Vol:4 No:11 2010Vol:4 No:10 2010Vol:4 No:09 2010Vol:4 No:08 2010Vol:4 No:07 2010Vol:4 No:06 2010Vol:4 No:05 2010Vol:4 No:04 2010Vol:4 No:03 2010Vol:4 No:02 2010Vol:4 No:01 2010
Vol:3 No:12 2009Vol:3 No:11 2009Vol:3 No:10 2009Vol:3 No:09 2009Vol:3 No:08 2009Vol:3 No:07 2009Vol:3 No:06 2009Vol:3 No:05 2009Vol:3 No:04 2009Vol:3 No:03 2009Vol:3 No:02 2009Vol:3 No:01 2009
Vol:2 No:12 2008Vol:2 No:11 2008Vol:2 No:10 2008Vol:2 No:09 2008Vol:2 No:08 2008Vol:2 No:07 2008Vol:2 No:06 2008Vol:2 No:05 2008Vol:2 No:04 2008Vol:2 No:03 2008Vol:2 No:02 2008Vol:2 No:01 2008
Vol:1 No:12 2007Vol:1 No:11 2007Vol:1 No:10 2007Vol:1 No:09 2007Vol:1 No:08 2007Vol:1 No:07 2007Vol:1 No:06 2007Vol:1 No:05 2007Vol:1 No:04 2007Vol:1 No:03 2007Vol:1 No:02 2007Vol:1 No:01 2007