Open Science Research Excellence

S Farajnia

Publications

2

Publications

2
6894
Application of Whole Genome Amplification Technique for Genotype Analysis of Bovine Embryos
Abstract:
In recent years, there has been an increasing interest toward the use of bovine genotyped embryos for commercial embryo transfer programs. Biopsy of a few cells in morulla stage is essential for preimplantation genetic diagnosis (PGD). Low amount of DNA have limited performing the several molecular analyses within PGD analyses. Whole genome amplification (WGA) promises to eliminate this problem. We evaluated the possibility and performance of an improved primer extension preamplification (I-PEP) method with a range of starting bovine genomic DNA from 1-8 cells into the WGA reaction. We optimized a short and simple I-PEP (ssI-PEP) procedure (~3h). This optimized WGA method was assessed by 6 loci specific polymerase chain reactions (PCRs), included restriction fragments length polymorphism (RFLP). Optimized WGA procedure possesses enough sensitivity for molecular genetic analyses through the few input cells. This is a new era for generating characterized bovine embryos in preimplantation stage.
Keywords:
Whole genome amplification (WGA), Genotyping, Bovine, Preimplantation genetic diagnosis (PGD)
1
14947
Inclusion of Enterococcus Faecalis and Enterococcus Faecium to UF White Cheese
Abstract:
Lighvan cheese is basically made from sheep milk in the area of Sahand mountainside which is located in the North West of Iran. The main objective of this study was to investigate the effect of enterococci isolated from traditional Lighvan cheese on the quality of Iranian UF white during ripening. The experimental design was split plot based on randomized complete blocks, main plots were four types of starters and subplots were different ripening durations. Addition of Enterococcus spp. did not significantly (P
Keywords:
Enterococcus faecalis, Enterococcus faecium,Lighvan cheese, Lipolysis, Proteolysis, UF cheese